Cand. Biol. Sc. (PhD), Kharkiv National University of Ukraine
Office: Science Building 277
Research Interests: avian genome mapping; molecular, population, conservation and evolutionary genetics.
Michael is interested in avian comparative genomics and physical mapping. He was involved in the genome projects for several avian species including the chicken, turkey, zebra finch, and California condor. In his research he employs high throughput BAC library screening to obtain sequence-ready large-insert clones harboring specific genes and/or markers - these can be used to obtain sequence information, as well as for physical and comparative mapping, and fluorescent in situ hybridization (FISH) assignment. The technique, which utilizes BAC filter hybridization with numerous overgo probes, has showed to be particularly fruitful for non-model species where detailed genome and sequence information are often lacking. For example, in the California condor (Gymnogyps californianus; Family Cathartidae), initiation of the genome project based on the BAC library resulted in the first chicken-condor comparative map that is now being integrated with the linkage and cytogenetic maps, and, eventually, will help us identify a cause for a genetic abnormality, lethal chondrodystrophy, in this highly endangered species.
Michael's current work on the white-throated sparrow (Zonotrichia albicollis) includes a combination of genomic and bioinformatics approaches to reveal the nature of distinct morphological, chromosomal and behavioral variation in this passerine bird.
In the past, Michael also worked in the field of population genetic studies, molecular genetics, and conservation in economically important avian species. He studied chicken genetic diversity and association with production traits using molecular genetic markers (e.g. protein polymorphisms, microsatellites, RAPDs, SNPs, PCR-RFLP) and techniques (e.g. BESS), and other inter-disciplinary approaches (e.g. morphological and molecular sexing markers in birds).
Michael has been involved in and maintains national and international collaborations on several genetic/genomic projects involving such organisms as a propionibacterium, wild rice, Garnett's bushbaby, American pika, chicken and other avian species.
Reprints for some publications are available as PDF files. By accessing the PDF file, the user agrees to abide by all copyright laws and education fair-use regulations.
Romanov MN, Dodgson JB, Gonser RA, Tuttle EM. Comparative BAC-based mapping in the white-throated sparrow, a novel behavioral genomics model, using interspecies overgo hybridization. BMC Res Notes. 2011; 4:211. Download PDF - (4.4 MB)
Blagoveshchenskiĭ IIu, Sazanova AL, Stekol'nikova VA, Fomichev KA, Barkova OIu, Romanov MN, Sazanov AA. [Investigation of pseudoautosomal and bordering regions in avian Z and W chromosomes with the use of large insert genomic BAC clones]. Genetika. 2011; 47(3):312-319.
MohammadabadiM R, Nikbakhti M, Mirzaee HR, Shandi MA, Saghi
DA, Romanov MN, Moiseyeva IG. Genetic variability in three native Iranian
chicken populations of the Khorasan Province based on microsatellite markers.
Genetika. 2010; 46 (4):572-576.
Romanov MN, Tuttle EM, Houck ML, Modi WS, Chemnick LG, Korody ML, Mork EM, Otten CA, Renner T, Jones KC, Dandekar S, Papp JC, Da Y; NISC Comparative Sequencing Program, Green ED, Magrini V, Hickenbotham MT, Glasscock J, McGrath S, Mardis ER, Ryder OA. The value of avian genomics to the conservation of wildlife. BMC Genomics. 2009; 10 (Suppl 2):S10.Download PDF - (1.4 MB)
Modi WS, Romanov M, Green ED, Ryder OA. Molecular cytogenetics of the California condor: evolutionary and conservation implications. Cytogenet Genome Res. 2009; 127(1):26-32. Download PDF - (2.1 MB)
Schmidt C, Romanov M, Ryder O, Magrini V, Hickenotham M, Glasscock J, Mardis E, McGrath S, Stein L. Gallus Genome GBrowse: a unified genomic database for the chicken. Nucleic Acids Res. 2008; 36 (Suppl 1):D719-723. Download PDF - (2.7 MB)
Romanov MN, Koriabine M, Nefedov M, de Jong PJ, Ryder OA. Construction of a California condor BAC library and first-generation chicken-condor comparative physical map as an endangered species conservation genomics resource. Genomics. 2006; 88:711-718. Download PDF - (849 kB)
Romanov MN, Dodgson JB. Cross-species overgo hybridization and comparative physical mapping within avian genomes. Anim Genet. 2006; 37:397-399. Download PDF - (55 KB)
International Chicken Genome Sequencing Consortium. Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution. Nature. 2004; 432:695-716. Download PDF - (1.4 MB)
Wallis JW, Aerts J, Groenen MA, Crooijmans RP, Layman D, Graves TA, Scheer DE, Kremitzki C, Fedele MJ, Mudd NK, Cardenas M, Higginbotham J, Carter J, McGrane R, Gaige T, Mead K, Walker J, Albracht D, Davito J, Yang SP, Leong S, Chinwalla A, Sekhon M, Wylie K, Dodgson J, Romanov MN, Cheng H, de Jong PJ, Osoegawa K, Nefedov M, Zhang H, McPherson JD, Krzywinski M, Schein J, Hillier L, Mardis ER, Wilson RK, Warren WC. A physical map of the chicken genome. Nature. 2004; 432:761-764. Download PDF - (131 KB)